A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry. (1st October 2020)

• Record Type: Journal Article
• Authors: Li, Ye; Liang, Jun; Shen, Yu; Kuang, Hai-Xue; Xia, Yong-Gang
• Is Part Of: Carbohydrate polymers. Volume 245(2020)
• Publication Date: 2020-10-01
• Subjects: ACN Acetonitrile -- AF Ammonium formateas -- Fuc D-fucose -- Gal D-galactose -- GalA D-galacturonic acid -- Glc D-glucose -- GlcA D-glucuronic acid -- Man D-mannose -- Rib D-ribose -- Xyl D-xylose -- ESI Electrospray ionization -- ESM External standard method -- FA Formic acid -- GC-MS Gas chromatography-mass spectrometry -- HPCE High-performance capillary electrophoresis -- HPLC High-performance liquid chromatography -- Ara L-arabinose -- LOD Limit of detection -- LOQ Limit of quantification -- Rha L-rhamnose -- QAMS Quantitative analysis of multi-monosaccharaides by single marker -- RCF Relative correction factor -- SIM Selective ion monitoring -- TCM Traditional Chinese medicine -- TFA Trifluoroacetic acid -- UHPLC-ESI-MS Ultra-high-performance liquid chromatography- electrospray mass spectrometry -- PMP 1-pheny-3-methyl-5-pyrazolone; Polysaccharide -- monosaccharide compositions -- UHPLC-ESI-MS -- QAMS; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116439
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: UHPLC-ESI-MS was applied for analysis of polysaccharides by acetylation. A quantitative analysis of multi-monosaccharaides by single marker was proposed. An analytical workflow was developed for UHPLC-ESI-MS and QAMS. Abstract: A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses. The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA). The separation was achieved on a Cortecs C18 + (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added. Furthermore, quantitative analysis of the multi-monosaccharaides by single marker (QAMS) was developed and validated using a relative correction factor. The UHPLC-ESI-MS and QAMS were compared and successfully applied to analyze the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica . The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.