Seawater transfer down-regulates C-type natriuretic peptide-3 expression in prolactin-producing cells of Japanese eel: Negative correlation with plasma chloride concentration. (1st May 2020)

• Record Type: Journal Article
• Authors: Katayama, Yukitoshi; Wong, Marty Kwok-Shing; Kusakabe, Makoto; Fujio, Megumi; Takahashi, Natsuki; Yaguchi, Miku; Tsukada, Takehiro
• Is Part Of: Molecular and cellular endocrinology. Volume 507(2020)
• Publication Date: 2020-05-01
• Subjects: CNP -- Osmoregulation -- Freshwater adaptation -- Euryhaline fish; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2020.110780
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: In euryhaline fishes, atrial and B-type natriuretic peptides are important hormones in hypo-osmoregulation, whereas osmoregulatory functions of C-type natriuretic peptides (CNPs) remain to be investigated. Although four CNP isoforms (CNP1-4) are mainly expressed in the brain, multiorgan expression of CNP3 was found in euryhaline Japanese eel, Anguilla japonica . Here we identified the CNP3-expressing cells and examined their response to osmotic stress in eel. CNP3 was expressed in several endocrine cells: prolactin-producing cells (pituitary), glucagon-producing cells (pancreas), and cardiomyocytes (heart). Pituitary CNP3 expression was the highest among organs and was decreased following seawater transfer, followed by a decrease in the freshwater-adaptating (hyper-osmoregulatory) hormone prolactin. We also showed the negative correlation between CNP3/prolactin expression in the pituitary and plasma Cl − concentration, but not for plasma Na + concentration. These results suggest that CNP3 in the pituitary (and pancreas) plays a critical role in freshwater adaptation of euryhaline eel together with prolactin. Highlights: CNP3 was expressed in several endocrine cells: prolactin-producing cells, glucagon-producing cells, and cardiomyocytes. Pituitary CNP3 was decreased by seawater transfer, followed by decrease in the freshwater-adaptating hormone prolactin. CNP3/prolactin expression in the pituitary is negatively correlated with plasma Cl - concentration.