Expression and function of DNAM‐1 on human B‐lineage cells. Issue 4 (29th November 2019)

• Record Type: Journal Article
• Authors: Nagayama‐Hasegawa, Yuko; Honda, Shin‐ichiro; Shibuya, Akira; Shibuya, Kazuko
• Is Part Of: Cytometry. Volume 98:Issue 4(2020)
• Page Start: 368
• Page End: 374
• Publication Date: 2019-11-29
• Subjects: DNAM‐1 (CD226) -- IL‐10 -- plasma cell -- plasmablast -- platelet; Flow cytometry -- Diagnostic use -- Periodicals; Cytodiagnosis -- Periodicals; 616.07582
• DOI: 10.1002/cyto.b.21859
• Languages: English
• ISSNs: 1552-4949

Abstract

Abstract: Background: Although DNAM‐1 is an activating receptor constitutively expressed on the majority of NK cells, CD8 + T cells, CD4 + T cells, monocytes, and platelets in human, several evidences demonstrated that a small population in B‐lineage cells also expressed DNAM‐1. However, the expression profile of DNAM‐1 on B‐lineage cells and its function remain obscure. Previous reports revealed that a considerable number of leukocytes including B cells in the peripheral blood conjugated to platelet. Thus, the proportion of DNAM‐1 + B‐lineage cells determined by flow cytometry analysis in the previous reports might be overestimated. Methods: We examined whether platelets conjugate B cells and then analyzed the expression of DNAM‐1 on the subpopulations of B‐lineage cells according to their maturation stages after exclusion of platelet‐conjugated B cells. We also assessed the involvement of DNAM‐1 in IL‐10 and antibody production from cultured B‐lineage cells stimulated with CpG‐ODN. Results: Approximately 10% of human DNAM‐1 + CD19 + B cells in the peripheral blood conjugated to platelets, resulting in the overestimation of the proportion of DNAM‐1 + B cells. After exclusion of platelet‐conjugating B cells, we show that DNAM‐1 expression was detected on subpopulations of memory B cells, plasmablasts, and plasma cells and upregulated by stimulation with CpG‐ODN. Moreover, DNAM‐1 was involved in IL‐10 and antibody productions by B cells after CpG‐ODN stimulation. Conclusions: DNAM‐1 may be involved in B‐lineage cell‐mediated immune responses.