UNIT 7.5 Overview of Digital Electrophoresis Analysis. Issue 9 (28th October 2014)

• Record Type: Journal Article
• Authors: Moomaw, Butch; Medberry, Scott; Gallagher, Sean R.
• Is Part Of: Current protocols essential laboratory techniques. Issue 9(2014)
• Page Start: 7.5.1
• Page End: 7.5.31
• Publication Date: 2014-10-28
• Subjects: electrophoresis -- imaging -- analysis -- CCD -- sCMOS -- 2‐D PAGE; Life sciences -- Laboratory manuals; Medical laboratories; Diagnosis, Laboratory; Clinical Laboratory Techniques; Clinical Protocols; Diagnosis, Laboratory; Medical laboratories; 570.28
• DOI: 10.1002/9780470089941.et0705s9
• Languages: English
• ISSNs: 1948-3430

Abstract

Abstract : Digital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using CCD‐based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of CCD technologies used for image capture, and provides an introduction to the software and methods used for one‐ and two‐dimensional digital image analysis. © 2014 by John Wiley & Sons, Inc.