UNIT 14E.6 Analysis of HSV Viral Reactivation in Explants of Sensory Neurons. Issue 1 (3rd November 2014)

• Record Type: Journal Article
• Authors: Arbuckle, Jesse H.; Turner, Anne‐Marie W.; Kristie, Thomas M.
• Is Part Of: Current protocols in microbiology. Volume 35:Issue 1(2014)
• Page Start: 14E.6.1
• Page End: 14E.6.21
• Publication Date: 2014-11-03
• Subjects: Herpes simplex virus -- latency model -- corneal infection -- trigeminal ganglion -- small‐molecule inhibitors -- viral titer -- immunohistochemistry; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/9780471729259.mc14e06s35
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract : As with all Herpesviruses, Herpes simplex virus (HSV) has both a lytic replication phase and a latency‐reactivation cycle. During lytic replication, there is an ordered cascade of viral gene expression that leads to the synthesis of infectious viral progeny. In contrast, latency is characterized by the lack of significant lytic gene expression and the absence of infectious virus. Reactivation from latency is characterized by the re‐entry of the virus into the lytic replication cycle and the production of recurrent disease. This unit describes the establishment of the mouse sensory neuron model of HSV‐1 latency‐reactivation as a useful in vivo system for the analysis of mechanisms involved in latency and reactivation. Assays including the determination of viral yields, immunohistochemical/immunofluorescent detection of viral antigens, and mRNA quantitation are used in experiments designed to investigate the network of cellular and viral proteins regulating HSV‐1 lytic infection, latency, and reactivation. © 2014 by John Wiley & Sons, Inc.