Dynamic profile for the detection of anti-SARS-CoV-2 antibodies using four immunochromatographic assays. Issue 2 (August 2020)

• Record Type: Journal Article
• Authors: Demey, Baptiste; Daher, Nagib; François, Catherine; Lanoix, Jean-Philippe; Duverlie, Gilles; Castelain, Sandrine; Brochot, Etienne
• Is Part Of: Journal of infection. Volume 81:Issue 2(2020)
• Page Start: e6
• Page End: e10
• Publication Date: 2020-08
• Subjects: SARS-CoV-2 -- COVID-19 -- Antibody -- Lateral flow assay; Infection -- Periodicals; Bacterial Infections -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.905
• DOI: 10.1016/j.jinf.2020.04.033
• Languages: English
• ISSNs: 0163-4453

Abstract

Abstract: In order to fight the SARS-CoV-2 pandemic infection, there is a growing need and demand for diagnostic tools that are complementary and different from the RT-PCR currently in use. Multiple serological tests are or will be very soon available but need to be evaluated and validated. We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2. In addition, we assessed the kinetics of antibody appearance using these assays in 22 patients after they were tested positive by RT-PCR. We observed great heterogeneity in antibody detection post-symptom onset. The median antibody detection time was between 8 and 10 days according to the manufacturers. All the tests showed a sensitivity of 60 to 80% on day 10 and 100% on day 15. In addition, a single cross-reaction was observed with other human coronavirus infections. Thus, immunochromatographic tests for the detection of anti-SARS-CoV-2 antibodies may have their place for the diagnostic panel of COVID-19.