Generation and Identification of Mutations Resulting in Chronic and Age‐Related Phenotypes in Mice. Issue 2 (21st May 2018)

• Record Type: Journal Article
• Authors: Blease, Andrew; Nicol, Thomas; Falcone, Sara; Starbuck, Becky; Greenaway, Simon; Hutchinson, Marie; Potter, Paul K.
• Editors: Auwerx, Johan; Ackerman, Susan L.; Brown, Stephen D.; Justice, Monica J.; Nadeau, Joseph
• Is Part Of: Current protocols in mouse biology. Volume 8:Issue 2(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-05-21
• Subjects: ageing -- age‐related disease -- mutagenesis -- phenotyping; Mice -- Genetics -- Laboratory manuals; Mice; Animals, Laboratory; Research; Mice -- Genetics; Periodicals; Laboratory manuals; 616.027333
• DOI: 10.1002/cpmo.42
• Languages: English
• ISSNs: 2161-2617

Abstract

Abstract: Aging is inevitable, and our society must deal with the consequences: namely, an increased incidence of disease and ill health. Many mouse models of disease are acute or early onset or are induced in young mice, despite the fact that aging is a significant risk factor for a range of significant diseases. To improve modeling of such diseases, we should incorporate aging into our models. Many systems are affected by aging, with a decline in mitochondrial function, an increase in senescence, a loss of resilience, telomere shortening, and a decline in immune function being key factors in the increased susceptibility to disease that is associated with aging. To develop novel models of age‐related disease, we undertook a phenotype‐driven screen of a pipeline of mutagenized mice. Here, we describe some of the underlying protocols and outline important aspects to consider when studying aged mice. © 2018 by John Wiley & Sons, Inc.