Nrf2 played an important role in radiation protection effect of low-level laser exposed on umbilical cord mesenchymal stem cell. (April 2020)

• Record Type: Journal Article
• Authors: Wang, Hong; Tu, Wen-Jun; Xiao, Changyan; Dong, Ming-Xin; Ye, Yin-Tao; Deng, Juan; Wang, Yan; Sha, Hong; Liu, Qiang
• Is Part Of: Tissue & cell. Volume 63(2020)
• Publication Date: 2020-04
• Subjects: Radiation protection -- Nrf2 -- LLLI; Cytology -- Periodicals; 571.5
• DOI: 10.1016/j.tice.2019.101329
• Languages: English
• ISSNs: 0040-8166

Abstract

Highlights: Low-level laser irradiation play role in ionizing irradiation protection function. LLLI treatment cells improved the results of erythrocyte count and myelopoiesis clones significantly. LLLI might improve the radiation protection effect through the molecular mechanisms relative with Nrf2 pathway in antioxidation. Abstract: To investigate the protective function of low-level laser irradiation (LLLI) against ionizing irradiation and explore the molecular mechanism of photomodulation of Nrf2 protein, the impact of LLLI (635 nm, 5.7 J/cm 2 ) before 2 Gy gamma ray radiation of radio-sensitive tissue hematopoietic stem cells was evaluated. As a result, reduced levels of reactive oxygen species and increased expression of antioxidant enzymes were detected. Moreover, increased expression of Nrf2 was observed after LLLI, whereas brusatol pretreatment before LLLI abolished this effect. In vivo, transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) was employed for therapy of hematopoietic function in an acute radiation sickness (H-ARS) mouse model, which was induced by 6-Gy ionizing irradiation; different hUC-MSC pretreatments including LLLI and Nrf2 RNAi were accounted for during experimental grouping. LLLI treatment of cells significantly increased the erythrocyte count and number of myelopoiesis clones ( P < 0.05), but such improvements were reduced by Nrf2 RNAi pretreatment compared with cells transplanted without intervention. Therefore, LLLI may improve the radiation protection effect through molecular mechanisms related to the Nrf2 antioxidant pathway.