Bioremediation of intertidal zones polluted by heavy oil spilling using immobilized laccase-bacteria consortium. (August 2020)

• Record Type: Journal Article
• Authors: Dai, Xiaoli; Lv, Jing; Yan, Guangxu; Chen, Chunmao; Guo, Shaohui; Fu, Pengcheng
• Is Part Of: Bioresource technology. Volume 309(2020)
• Publication Date: 2020-08
• Subjects: Intertidal zones -- Heavy oil pollution -- Bioremediation -- Immobilized laccase-bacteria consortium; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123305
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Immobilized laccase-bacteria consortium was used to remediate heavy oil pollution. A coastal and open intertidal zone simulation system was constructed. Long chain n-alkanes of C26-C35 were effectively degraded. Polycyclic aromatic hydrocarbons with more than 3-ring were effectively degraded. Obligate hydrocarbon degrading bacteria were analyzed. Abstract: Heavy oil pollution in the intertidal zones has become a worldwide environmental problem. In this study, bioremediation on heavy oil pollutants in the intertidal zones using an immobilized laccase-bacteria consortium system was evaluated with the aid of intertidal experimental pools built in the coastal area. It is found that degradation efficiency of the immobilized laccase-bacteria consortium for heavy oil was 66.5% after 100 days remediation, with the reaction rate constant of 0.018 d −1 . Gas Chromatograph-Mass Spectrometer analysis shows that degradation efficiency of saturated hydrocarbons and aromatic hydrocarbons were 79.2% and 78.7%, which were 64.9% and 65.1% higher than control. It is further seen that degradation of long-chain n-alkanes of C26-C35 and polycyclic aromatic hydrocarbons with more than three rings were significant. Metagenomic analysis indicates that the immobilized laccase-bacterial consortium has not only increased the biodiversity of heavy oil degrading bacteria, but also accelerated the degradation of heavy oil.