Ameliorative effect of low molecular weight peptides from the head of red shrimp (Solenocera crassicornis) against cyclophosphamide-induced hepatotoxicity in mice. (September 2020)

• Record Type: Journal Article
• Authors: Jiang, Shuoqi; Zhang, Zhuangwei; Yu, Fangmiao; Zhang, Zhoufeng; Yang, Zuisu; Tang, Yunping; Ding, Guofang
• Is Part Of: Journal of functional foods. Volume 72(2020)
• Publication Date: 2020-09
• Subjects: Solenocera crassicornis -- Cyclophosphamide -- Hepatotoxicity -- Low molecular weight peptides; CTX Cyclophosphamide -- MW Molecular Weight -- ROS Reactive oxygen species -- ALT Alanine aminotransferase -- AST Aspartate aminotransferase -- AKP Alkaline phosphatase -- CYP 450 Cytochrome P450 -- Nrf2 Nuclear factor erythroid-2-related factor 2 -- Keap1 Kelch-like ECH-associated protein 1 -- CAT Catalase -- T-AOC Total antioxidant capacity -- GSH-Px Glutathione peroxidase -- SOD Superoxide Dismutase -- MDA Malondialdehyde -- GCLM Glutamate cysteine ligase modifier subunit -- HO-1 Heme oxygenase 1 -- NQO-1 NADPH Quinone acceptor Oxidoreductase 1 -- NF-κB Nuclear factor kappa-B -- IKK Inhibitor of nuclear factor kappa-B kinase -- IκB Inhibitor of NF-κB -- 6IL-1β, 6 Interleukin-1β -- IFN-γ Interferon-γ -- TNF-α Tumor necrosis factor-α; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104085
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Low molecular-weight peptides were prepared from Solenocera crassicornis head. SCHPs-F1 ameliorated hepatotoxicity induced by cyclophosphamide in mice. SCHPs-F1 restored endogenous antioxidants levels by activating the Nrf2 signal. SCHPs-F1 suppressed hepatic inflammation through NF-κB signal pathway. SCPHs-F1 relieved CTX hepatotoxicity as functional component. Abstract: Our study was aimed at investigating the protective effect of low molecular weight peptides (SCHPs-F1) from red shrimp ( Solenocera crassicornis) head against cyclophosphamide-induced hepatotoxicity in mice. We found that SCHPs-F1 treatment dose-dependently normalized the biochemical markers, hepatic index, and total CYP450 enzyme content in CTX-induced hepatotoxicity in mice. SCHPs-F1 also ameliorated the CTX-mediated structural disorders of the hepatic tissue. Western blot results suggested that SCHPs-F1 significantly restored the levels of endogenous antioxidants (CAT, T-AOC, GSH-Px, SOD and MDA levels) through activating the Nrf2 signal by upregulating the expression of GCLM, HO-1, and NQO-1. Moreover, SCHPs-F1 improved the CTX-induced hepatotoxicity by inhibiting of NF-κB signal responses and down-regulating the expression of the inflammatory factors (IL-1β, IL-6, IFN-γ and TNF-α). These findings suggest that SCHPs-F1 can regulate Nrf2 and NF-κB signaling pathways and reduce the oxidative stress and inflammation in CTX-induced hepatotoxicity. Overall, SCHPs-F1 are value-added food ingredients for alleviating CTX-induced hepatotoxicity.