Daily consumption of black soybean (Glycine max L.) seed coat polyphenols attenuates dyslipidemia in apolipoprotein E-deficient mice. (September 2020)

• Record Type: Journal Article
• Authors: Tanaka, Wataru; Matsuyama, Hiroki; Yokoyama, Daigo; Yamashita, Yoko; Ashida, Hitoshi; Sakono, Masanobu; Sakakibara, Hiroyuki
• Is Part Of: Journal of functional foods. Volume 72(2020)
• Publication Date: 2020-09
• Subjects: apolipoprotein E-deficient mice -- Black soybean (Glycine max L.) seed coat -- Cyanidin-3-glucoside -- Dyslipidaemia -- Cholesterol -- Triglyceride; ApoE−/− apolipoprotein E-deficient -- BE black soybean seed coat extract -- CN AIN-93M based control diet used in this study -- Cyp7a1 cholesterol 7α-hydroxylase -- EGCG epigallocatechin-3-gallate -- Fasn fatty acid synthase -- GIP glucose-dependent insulinotropic polypeptide -- Pparα peroxisome proliferator-activated receptor α; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104054
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Daily consumption of black soybean seed coat polyphenols attenuates dyslipidemia in apolipoprotein E-deficient mice. Highlights: Black soybean seed coat extract (BE) rich in polyphenols, such as cyanidin glucoside. Apolipoprotein E-deficient (ApoE −/− ) mice spontaneously develop dyslipidemia. BE lowered serum and hepatic total cholesterol and triglyceride levels in ApoE −/− mice. These were characteristic in ApoE −/− mice, but not in their corresponding wild-type. Abstract: Black soybean ( Glycine max L.) seed coat extract (BE) is rich in polyphenols, including epicatechin, procyanidin, and cyanidin-3-glucoside. We evaluated the effects of daily consumption of BE in apolipoprotein E-deficient (ApoE −/− ) mice. ApoE −/− and wild-type BALB/c mice were fed a 20% fat diet with or without 1% BE for 8 weeks. Bodyweight was unaffected by BE. BE supplementation significantly lowered serum and hepatic total cholesterol and hepatic triglyceride levels. Hepatic mRNA expression of cholesterol 7α-hydroxylase ( Cyp7a1 ) and peroxisome proliferator-activated receptor α ( Pparα ) were significantly stimulated in ApoE −/− mice consuming the BE diet. These effects were characteristic in ApoE −/− mice, but not in their corresponding wild type. In conclusion, it is clear that dietary consumption of BE affected blood and hepatic levels of cholesterol and triglyceride in ApoE −/− mice, and consequently exerted preventive effects for dyslipidemia. Putative mechanisms include the upregulation of hepatic Cyp7a1 and Pparα mRNA expression.