Renoprotective effect of stevia residue extract on adenine-induced chronic kidney disease in mice. (September 2020)

• Record Type: Journal Article
• Authors: Mehmood, Arshad; Zhao, Liang; Ishaq, Muhammad; Zad, Oumeddour Dounya; Zhao, Lei; Wang, Chengtao; Usman, Muhammmad; Lian, Yunhe; Xu, Meili
• Is Part Of: Journal of functional foods. Volume 72(2020)
• Publication Date: 2020-09
• Subjects: Stevia residue extract -- Adenine -- Chronic kidney disease -- Hyperuricaemia -- Gut microbiota; chronic kidney disease CKD -- STRE stevia residue extract -- MCP-1 monocyte chemoattractant protein-1 -- GSH reduced glutathione -- T-AOC total antioxidant capacity -- MDA malondialdehyde -- SOD superoxide dismutase -- CAT catalase -- BUN blood urea nitrogen -- NGAL neutrophil gelatinase-associated lipocalin -- ADA adenine deaminase -- XOD xanthine oxidase -- TNF-α Tumor necrosis factor-alpha -- IL-1β Interleukin 1 beta IL-6, Interleukin 6 -- IL-10 Interleukin 1 10 -- URAT1 urate transporter-1 (URAT1) -- GLUT9 glucose transporter 9 -- CMC-Na Sodium salt of carboxymethylcellulose -- H & E haematoxylin and eosin -- PAS Periodic acid-Schiff -- MAS Masson's trichrome -- SEM scanning electron microscope -- TEM Transmission electron microscopy -- IHC immunohistochemistry -- HO-1 haem oxygenase-1 -- ECM extracellular matrix; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.103983
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: STRE markedly improve oxidative, inflammatory related biomarkers and renal structure. STRE attenuated renal fibrosis in adenine-induced CKD mice. STRE modulate gut microbiota composition at some extent in adenine induced CKD mice. Abstract: The present study was performed to explore the renoprotective potential of a stevia residue extract (STRE) on a mouse model of adenine-induced chronic kidney disease (CKD). The mice were divided into five groups (n = 10): control, model, Po, STRE-1, and STRE-2. Adenine (0.20% in the normal diet for 3 weeks) was used to induce CKD. Allopurinol and STRE markedly restored the balance of functional, oxidative, and inflammatory biomarkers and improved the renal histopathology and ultrastructure compared to the Mod group. Moreover, STRE also attenuated the renal fibrosis by suppressing the TGF-β1/Smad and Wnt/β-catenin signalling pathways and improved the gut microbiota composition. The results of present study confirmed that STRE has a potential to attenuate renal injury caused by adenine and may be used to develop of nutraceutical products, warranting further studies to elucidate beneficial effects of STRE.