Overexpression of SFA1 in engineered Saccharomyces cerevisiae to increase xylose utilization and ethanol production from different lignocellulose hydrolysates. (October 2020)

• Record Type: Journal Article
• Authors: Zhu, Lang; Li, Pengsong; Sun, Tongming; Kong, Meilin; Li, Xiaowei; Ali, Sajid; Liu, Wenbo; Fan, Sichun; Qiao, Jingchun; Li, Shizhong; Peng, Liangcai; He, Boyang; Jin, Mingjie; Xiao, Wei; Cao, Limin
• Is Part Of: Bioresource technology. Volume 313(2020)
• Publication Date: 2020-10
• Subjects: Lignocellulosic -- Ethanol -- Xylose -- SFA1 -- Saccharomyces cerevisiae; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123724
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Ethanol yields of SFA1 OE were around 0.492 g/g total sugars in different hydrolysates. Diploid strain SQ-2 displays improved ethanol yield and high temperature resistance. Contributions of gene SFA1 on ethanol yields were evaluated in various hydrolysates. SFA1 OE with high ethanol yield fits to alkaline-distilled sweet sorghum bagasse. Abstract: Here, an engineered Saccharomyces cerevisiae strain SFA1 OE was constructed by overexpressing SFA1 in a reported WXY70 with effective six-gene clusters. Under simulated maize hydrolysate, SFA1 OE produced an ethanol yield of 0.492 g/g total sugars within 48 h. The productivity of SFA1 OE was comprehensively evaluated in typical hydrolysates from stalks of maize, sweet sorghum, wheat and Miscanthus . Within 48 h, SFA1 OE achieved an ethanol yield of 0.489 g/g total sugars in the optimized hydrolysate of alkaline-distilled sweet sorghum bagasse derived from Advanced Solid-State Fermentation process. By crossing SFA1 OE with a DQ1-derived haploid strain, we obtained an evolved diploid strain SQ-2, exhibiting improved ethanol production and thermotolerance. This study demonstrates that overexpressing SFA1 enables efficient fermentation performance in different lignocellulosic hydrolysates, especially in the hydrolysate of alkaline-distilled sweet sorghum bagasse. The increased cellulosic bioethanol production of SFA1 OE provides a promising platform for efficient biorefineries.