Bioethanol production from the comparison between optimization of sorghum stalk and sugarcane leaf for sugar production by chemical pretreatment and enzymatic degradation. (15th October 2020)

• Record Type: Journal Article
• Authors: Manmai, Numchok; Unpaprom, Yuwalee; Ponnusamy, Vinoth Kumar; Ramaraj, Rameshprabu
• Is Part Of: Fuel. Volume 278(2020)
• Publication Date: 2020-10-15
• Subjects: Agriculture wastes -- Chemical pretreatment -- Enzymatic hydrolysis and Bioethanol production -- Central composite design (CCD); Fuel -- Periodicals; Coal -- Periodicals; Coal; Fuel; Periodicals; 662.6
• DOI: 10.1016/j.fuel.2020.118262
• Languages: English
• ISSNs: 0016-2361

Abstract

Graphical abstract: Highlights: Agricultural wastes are one of renewable and low-cost biomass for biofuel. This study conducted using bioethanol production from sorghum stalk and sugarcane leaf. Total sugar was optimized by the response surface methodology on central composite design. Enzymatic hydrolysis was achieved at 24 hr of hydrolysis efficiency. Abstract: This work reports the comparison of optimized pretreatment models on total sugar (TS) production from sugarcane leaf (SL) with sorghum stalk (SS) utilization to decompose the lignocellulosic biomass into oligosaccharide (OS). The accumulation variables were examined in the pretreatment model, including pretreatment time (1–3 days), concentrations of sodium hydroxide (NaOH) from 1 to 2% (w/v), and heating temperatures from 30 to 40 °C. TS productions were optimized by using response surface methodology (RSM) on central composite design (CCD) models. These models indicated excellent determination coefficients (R 2 ) ranged from 0.9820 to 0.9900 and are significant. Process optimization showed that the highest TS yield was achieved of 32.612 g/L and 38.977 g/L for NaOH pretreatment at condition (2)% (w/v) NaOH at the temperature of 40 °C for 3 days. After pretreatment, the biomass was degraded using a cellulase enzyme to form the fermentable sugar for ethanol production by Saccharomyces cerevisiae TISTR5020. The maximum bioethanol yield of 8.374 ± 1.813 g/L was achieved at 2 days of fermentation time when compared with bioethanol yield (5.234 ± 0.907 g/L) of 4 days fermentation time.