The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia. (2020)

• Record Type: Journal Article
• Authors: Sakaguchi, Masahiro; Nakajima, Nana; Yamaguchi, Hiroki; Najima, Yuho; Shono, Katsuhiro; Marumo, Atsushi; Omori, Ikuko; Fujiwara, Yusuke; Terada, Kazuki; Yui, Shunsuke; Wakita, Satoshi; Mitaya, Miho; Arai, Kunihito; Kitano, Tomoaki; Doki, Noriko; Ohashi, Kazuteru; Inokuchi, Koiti
• Is Part Of: Leukemia research reports. Volume 13(2020)
• Publication Date: 2020
• Subjects: FLT3-ITD -- Allelic ratio -- Detection method -- Sensitivity -- Acute myeloid leukemia; Leukemia -- Periodicals; Lymphomas -- Periodicals; Multiple myeloma -- Periodicals; Leukemia -- Periodicals; Lymphoma -- Periodicals; Multiple Myeloma -- Periodicals; Hematologic Neoplasms -- Periodicals; Leukemia; Lymphomas; Multiple myeloma; Electronic journals; Periodicals; 616.9941905
• DOI: 10.1016/j.lrr.2020.100198
• Languages: English
• ISSNs: 2213-0489

Abstract

Abstract: Fms-like tyrosine kinase 3 -internal tandem duplication ( FLT3 -ITD) is a key predictive factor for the prognosis of acute myeloid leukemia (AML). We compared the detection sensitivity of fragment analysis with that of PCR-electrophoresis using MV4-11 ( FLT3 -ITD) and NKM-1 ( FLT3 -wild type) cell lines. DNA of these cells was mixed at different ratios and subjected to PCR-electrophoresis or fragment analysis. PCR-electrophoresis was found to have an FLT3 -ITD allelic ratio (AR) detection limit of 0.034–0.072. Visual inspection of the PCR-electrophoresis revealed a lower detection sensitivity than that of fragment analysis. Therefore, it is essential to conduct fragment analysis when screening for FLT3 -ITD.