Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways. (August 2020)

• Record Type: Journal Article
• Authors: Jia, Yangyang; Li, Xin; Nan, Aruo; Zhang, Nan; Chen, Lijian; Zhou, Hanyu; Zhang, Han; Qiu, Miaoyun; Zhu, Jialu; Ling, Yihui; Jiang, Yiguo
• Is Part Of: Environment international. Volume 141(2020)
• Publication Date: 2020-08
• Subjects: PM2.5 -- Circ_406961 -- Inflammatory reaction -- ILF2 -- STAT3/JNK pathways; Environmental protection -- Periodicals; Environmental health -- Periodicals; Environmental monitoring -- Periodicals; Environmental Monitoring -- Periodicals; Environnement -- Protection -- Périodiques; Hygiène du milieu -- Périodiques; Environnement -- Surveillance -- Périodiques; Environmental health; Environmental monitoring; Environmental protection; Periodicals; 333.705
• DOI: 10.1016/j.envint.2020.105755
• Languages: English
• ISSNs: 0160-4120

Abstract

Highlights: CircRNA interact with protein to regulate the PM2.5 -induced inflammatory responses. Circ_406961 inhibit PM2.5 -induced BEAS-2B cells inflammatory responses. ILF2 affect inflammatory response via STAT3/JNK signal pathway. Abstract: Fine particulate matter (PM2.5 ) has been verified to augmented the incidence of pneumonia, asthma, pulmonary fibrosis, and other pulmonary diseases. Airway inflammation is the pathological basis of the respiratory system, and understanding the molecular mechanisms responsible for airway inflammation may thus support the diagnosis and treatment of respiratory diseases. In our study, human bronchial epithelial cells (BEAS-2B) were exposed to various concentrations of PM2.5 for 48 h. PM2.5 entered the cells, resulting in increased production of interleukin 6 (IL-6) and interleukin 8 (IL-8) and decreased the expression of circular RNA 406961 (circ_406961). Further, PM2.5 with a concentration of 75 μg/mL was applied to mechanism study. Functional experiments further confirmed that circ_406961 inhibited PM2.5 -induced BEAS-2B cell inflammation. RNA pull-down and mass spectrometry showed that circ_406961 interacted with interleukin enhancer-binding factor 2 (ILF2), which could regulate phosphorylation of signal transducer and activator of transcription 3 (STAT3) and mitogen-activated protein kinase 8 (MAPK8, JNK). Our studies showed that circ_406961 inhibited activation of STAT3/JNK pathways via interacting with ILF2 protein, thereby inhibiting the PM2.5 -induced inflammatory reaction.