Nutrient metabolism, mass balance, and microbial structure community in a novel denitrifying phosphorus removal system based on the utilizing rules of acetate and propionate. (October 2020)

• Record Type: Journal Article
• Authors: Zhang, Miao; Zhu, Chenjie; Pan, Ting; Fan, Yajun; Soares, Ana; Wu, Jun; He, Chengda
• Is Part Of: Chemosphere. Volume 257(2020)
• Publication Date: 2020-10
• Subjects: A2/O -- MBBR -- Carbon source -- Denitrifying phosphorus removal -- Mass balance -- Illumina MiSeq sequencing -- Operation optimization; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.127076
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The effect of acetate (HAc) and propionate (HPr) on denitrifying phosphorus removal (DPR) was evaluated in a novel two-sludge A 2 /O - MBBR (anaerobic/anoxic/oxic - moving bed biofilm reactor) system. Results showed that it was the carbon source transformation and utilization especially the composition of poly-β-hydroxyalkanoates (PHA) (mainly poly-β-hydroxybutyrate (PHB) and poly-bhydroxyvalerate (PHV)) decided DPR performance, where the co-exist of HAc and HPr promoted the optimal nitrogen (85.77%) and phosphorus (91.37%) removals. It facilitated the balance of PHB and PHV and removing 1 mg NO3 − (PO4 3− ) consumed 3.04–4.25 (6.84–9.82) mgPHA, where approximately 40–45% carbon source was saved. Mass balance revealed the main metabolic pathways of carbon (MAn, C (consumed amount in anaerobic stage) and MA-O, C (consumed amount in anoxic and oxic stages): 66.38–76.19%), nitrogen (MDPR, N (consumed amount in DPR): 57.01–65.75%), and phosphorus (MWS, P (discharged amount in waste sludge): 81.05–85.82%). Furthermore, the relative abundance and microbial distribution were assessed to elucidate DPR mechanism (e.g. Accumulibacter, Acinetobacter, Dechloromonas, Competibacter, and Defluviicoccus ) in the A 2 /O reactor and nitrification performance (e.g. Nitrosomonas, Nitrosomonadaceae and Nitrospira ) in the MBBR. Carbon source was demonstrated as the key point to stimulate the biodiversity and bioactivity related to DPR potential, and the operational strategy of carbon source addition was proposed based on the utilizing rules of HAc and HPr. Graphical abstract: Image 1 Highlights: It was feasible to strengthen DPR with the co-exist of acetate and propionate. Carbon source revealed the strong relation with MAn, C efficiency and PHB/PHA ratio. 40–45% carbon addition can be saved by the efficient utilization of carbon source. Mass balance provided theoretical reference for the nutrient metabolic pathways. Carbon source promoted the shift of species diversity and functional bacteria.