Effects of Agaricus blazei polypeptide on cell senescence by regulation of Keap1/Nrf2/ARE and TLR4/NF-κBp65 signaling pathways and its mechanism in D-gal-induced NIH/3T3 cells. (September 2020)

• Record Type: Journal Article
• Authors: Feng, Qingxia; Lu, Xuechun; Yuan, Guangxin; Zhang, Qian; An, Liping
• Is Part Of: Journal of functional foods. Volume 72(2020)
• Publication Date: 2020-09
• Subjects: Agaricus blazei -- ABp-RQR -- Aging -- Nrf2/ARE -- TLR4/NF-κBp65; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104037
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Three active components ABp-RRQ, ABp-RQR and ABp-TRAR were obtained. ABp-RQR was the main active component to alleviate the oxidative inflammatory reactions. ABp-RQR play an anti-aging role by regulating Nrf2/ARE and TLR4/NF-κBp65 pathways. Abstract: Agaricus blazei is a kind of fungus plant with an edible as well as a medicinal use. Previous experiments have shown its crude peptide to possess an obvious anti-aging effect. In the present study three active components viz . ABp-RRQ, ABp-RQR and ABp-TRAR were obtained from the Agaricus blazei polypeptide. ABp-RQR was the main active component with a molecular weight of 458 Dalton, and HPLC confirmed it's a purity > 98%. When the D-galactose(D-gal)-induced NIH/3T3 cells were treated with ABp-RQR, changes were observed in the oxidative stress activities and the contents of inflammatory factors. Cell morphology and the percentage of positive cells were observed using the β-galactosidase staining. Western blot was used to detect the expression levels of the proteins modulated in the inflammatory and antioxidant signaling pathways. Our study suggests that ABp-RQR may play an anti-aging role by alleviating the oxidative inflammatory reactions in D-gal-induced NIH/3T3 cells.