Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways. (September 2020)

• Record Type: Journal Article
• Authors: Zhang, Jing; Wang, Wei; Mao, Xiangzhao
• Is Part Of: Journal of functional foods. Volume 72(2020)
• Publication Date: 2020-09
• Subjects: Antioxidant -- Chitopentaose hydrochloride -- H2O2 -- HaCaT cells -- MAPKs pathway -- Nrf2/ARE pathway; HaCaT Human keratinocyte cell line -- COSs chito-oligosaccharides -- COS-3 Chitotriose hydrochloride -- COS-5 Chitopentaose hydrochloride -- COS-7 Chitoheptaose hydrochloride -- MTT thiazolyl blue tetrazolium bromide -- ROS reactive oxygen species -- DCFH-DA 2, 7-dichlorodi-hydroflfluorescein diacetate -- H2O2 hydrogen peroxide -- ELISA enzyme linked immunosorbent assay -- SOD superoxide dismutase -- CAT Catalase -- MDA malondialdehyde -- LDH lactate dehydrogenase -- JC-1 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolyl carbocyanine iodide -- MMP mitochondrial membrane potential -- MAPKs mitogen-activated protein kinases -- Nrf2 Nuclear factor E2 related factor 2 -- ARE antioxidant response element; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104086
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: COS-5 can increase cell viability and reduce ROS content. COS-5 can regulate anti-oxidant enzymes system. COS-5 can inhibit the MMP and the expression of Caspase3. COS-5 can regulate MAPKs and Nrf2/ARE pathways. Abstract: Chito-oligosaccharides, the novel marine functional oligosaccharides, possess broad activities including anti-inflammatory and anti-oxidative effects. However, there are few studies about the molecular mechanisms of chito-oligosaccharides monomers against oxidative stress. In this study, the anti-oxidative effect and mechanism of Chitopentaose hydrochloride (COS-5) were explored in HaCaT cells. The results indicated that COS-5 improved cell viability and decreased the production of ROS induced by hydrogen peroxide (H2 O2 ). COS-5 can increase the antioxidant-related enzymes including SOD and CAT and can reduce MDA production and LDH leakage in HaCaT cells. COS-5 effectively inhibited the decline of MMP and the expression of Caspase-3 protein. Moreover, COS-5 can significantly down-regulate abnormal activation of MAPKs pathway and up-regulate the Nrf2/ARE pathway. In summary, COS-5 has significant protective effect against H2 O2 -induced oxidative stress in HaCaT cells through regulating MAPKs and Nrf2/ARE pathways, suggesting that COS-5 may be used as a novel functional food supplement to prevent oxidative-stress-related diseases in the future.