Immunoglobulin light chain κ precedes λ rearrangement in swine but a majority of λ+ B cells are generated earlier. (October 2020)

• Record Type: Journal Article
• Authors: Sinkora, Marek; Stepanova, Katerina; Sinkorova, Jana
• Is Part Of: Developmental and comparative immunology. Volume 111(2020)
• Publication Date: 2020-10
• Subjects: Porcine immune system -- B cell development -- B cell receptors -- Immunoglobulins -- Cell differentiation -- Other animals; Ab(s) antibody(ies) -- BCR B cell receptor -- BM bone marrow -- CDR3 complementary determining region 3 -- Ig immunoglobulin -- IgH immunoglobulin heavy chain -- KDE kappa deleting element -- IgL immunoglobulin light chain -- MHC-II major histocompatibility class II antigen -- PBS phosphate buffer solution -- SLC surrogate light chain -- VDJH complete VDJ rearrangement for immunoglobulin heavy chain -- VJL complete VJ rearrangement for immunoglobulin light chain -- VpreB invariable immunoglobulin iota chain of SLC (CD179a); Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2020.103751
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Developmental pathways for B cell lymphogenesis are sufficiently known only in mice and humans. However, both of these species rearrange immunoglobulin heavy chains (IgH) before light chains (IgL) while IgL precedes IgH rearrangement in swine. We demonstrate here that this reversed order of rearrangements have some concealed consequences: (1) we confirmed that although IgLκ rearrangement is initial, most IgLλ + B cells are generated earlier and before IgH rearrangements, while most IgLκ + B cells later and after IgH rearrangements, (2) the second IgLκ rearrangement can occur after IgLλ rearrangement, (3) early formed B cells bear only single in-frame IgH rearrangements, (4) many IgLκ + B cells carry IgLλ rearrangements that can be productive and occurring on both alleles in one cell, and (5) although VpreB and λ5 genes are present in swine, they are preferentially expressed in non-B cells. In summary, our findings reveal that swine use an alternative B cell developmental pathway as compared to mice and humans. Highlights: Initial IgLκ rearrangement is replaced by IgLλ rearrangement before IgH rearrangement . Most IgLλ + B cells are generated earlier while most IgLκ + B cells later. Eearly formed B cells bear only single IgH rearrangements. Many IgLκ + B cells carry IgLλ rearrangements that can be productive and multiple. VpreB and λ5 genes are present in swine but they are mostly expressed in non-B cells.