Aseptic Technique. Issue 1 (9th March 2020)

• Record Type: Journal Article
• Authors: Bykowski, Tomasz; Stevenson, Brian
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 56:Issue 1(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-03-09
• Subjects: aseptic technique -- bacteriology -- Bunsen burner -- cell culture -- laminar flow -- laminar hood -- sterilization -- virology; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.98
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: This article describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as "aseptic technique." Two major strategies for aseptic work are described: using a Bunsen burner and using a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes. © 2020 by John Wiley & Sons, Inc.