Issue Information. Issue 2 (7th June 2020)

• Record Type: Journal Article
• Is Part Of: Current protocols in chemical biology. Volume 12:Issue 2(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-06-07
• Subjects: Biochemistry -- Laboratory manuals; Chemical Phenomena; Biochemistry; Periodicals; Laboratory manuals; 572.078
• DOI: 10.1002/cpch.67
• Languages: English
• ISSNs: 2160-4762

Abstract

Abstract : Cover : In Knutson and Heemstra (https://doi.org/10.1002/cpch.82 ): (A ) A‐to‐I RNA editing is the result of a site‐selective deamination reaction catalyzed by ADAR enzymes. (B ) A‐to‐I editing alters base pairing properties of the nucleobase, resulting in I:C hydrogen bonding. (C ) Crystal structure of EndoV (PDB 2W35) bound to a single‐stranded DNA substrate (purple) with inosine (red) and a Mg 2+ ion (blue). (D ) EndoV supplemented with Mg 2+ results in cleavage of inosine containing transcripts, while Ca 2+ promotes binding. (E) General concept of immunoprecipitation using E. coli EndoV (eEndoV)—maltose binding protein (MBP) fusion and anti‐MBP magnetic beads.