Exploration of Energy Metabolism in the Mouse Using Indirect Calorimetry: Measurement of Daily Energy Expenditure (DEE) and Basal Metabolic Rate (BMR). Issue 3 (1st September 2015)

• Record Type: Journal Article
• Authors: Meyer, Carola W.; Reitmeir, Peter; Tschöp, Matthias H.
• Is Part Of: Current protocols in mouse biology. Volume 5:Issue 3(2015)
• Page Start: 205
• Page End: 222
• Publication Date: 2015-09-01
• Subjects: daily energy expenditure -- indirect calorimetry -- mouse phenotyping -- basal metabolic rate; Mice -- Genetics -- Laboratory manuals; Mice; Animals, Laboratory; Research; Mice -- Genetics; Periodicals; Laboratory manuals; 616.027333
• DOI: 10.1002/9780470942390.mo140216
• Languages: English
• ISSNs: 2161-2617

Abstract

Abstract : Current comprehensive mouse metabolic phenotyping involves studying energy balance in cohorts of mice via indirect calorimetry, which determines heat release from changes in respiratory air composition. Here, we describe the measurement of daily energy expenditure (DEE) and basal metabolic rate (BMR) in mice. These well‐defined metabolic descriptors serve as meaningful first‐line read‐outs for metabolic phenotyping and should be reported when exploring energy expenditure in mice. For further guidance, the issue of appropriate sample sizes and the frequency of sampling of metabolic measurements is also discussed. © 2015 by John Wiley & Sons, Inc.