Characterization of a new biosurfactant produced by an effective pyrene-degrading Achromobacter species strain AC15. (August 2020)

• Record Type: Journal Article
• Authors: Li, Jing; Wang, Yao; Zhou, Wei; Chen, Weixin; Deng, Maocheng; Zhou, Shaoqi
• Is Part Of: International biodeterioration & biodegradation. Volume 152(2020)
• Publication Date: 2020-08
• Subjects: Achromobacter species -- Lipopeptide -- Pyrene -- High-molecular weight polycyclic aromatic hydrocarbons -- Biodegradation -- Characterization; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2020.104959
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: An Achromobacter species strain AC15 was isolated from mangrove soil and identified as a potent producer of biosurfactant. It could utilize high concentrations of pyrene as the sole source of carbon and energy. Results showed that AC15 degraded about 40% of the pyrene, which was provided at an initial concentration of 300 mg L −1, and reduced surface tension of the culture medium from 67.2 to 33.2 mN m −1 after 14 d of incubation using its good emulsification property on the culture medium. The main chemical component of partially purified biosurfactant, namely BS15, was analyzed by gas chromatography-mass spectrometry, amino acid analysis, and LTQ Orbitrap Elite mass spectrometry. Results showed that BS15 was a novel linear lipopeptide with a long-chain C16 fatty acid and peptide comprised of four amino acid residues. The molecular structure was determined as CH3 -(CH2 )14 –CO–O-Leu-Asp-Lys-Ser. The solubility of pyrene in water improved 1.5–1.9 times in the presence of 40–60 mg L −1 of BS15. This work demonstrated that strain AC15 and its biosurfactant BS15 could be potential candidates in further applications of bioremediation of high-molecular weight polycyclic aromatic hydrocarbons. Highlights: Achromobacter species strain AC15 isolated from mangrove soil could utilize pyrene as the sole source of carbon. This strain was capable of producing biosurfactant with potential for bioremediation. A new linear lipopeptide BS15 was obtained with a molecular structure of CH3 -(CH2 )14 –CH2 –CO-Leu-Asp-Lys-Ser. BS15 showed good interfacial and emulsification characteristics.