UNIT 6G.2 Genetic Manipulation of Acinetobacter baumannii. Issue 1 (3rd November 2014)

• Record Type: Journal Article
• Authors: Jacobs, Anna C.; Thompson, Mitchell G.; Gebhardt, Michael; Corey, Brendan W.; Yildirim, Suleyman; Shuman, Howard A.; Zurawski, Daniel V.
• Is Part Of: Current protocols in microbiology. Volume 35:Issue 1(2014)
• Page Start: 6G.2.1
• Page End: 6G.2.11
• Publication Date: 2014-11-03
• Subjects: Acinetobacter baumannii -- mutagenesis -- conjugation -- transformation -- electroporation -- plasmid; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/9780471729259.mc06g02s35
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract : Acinetobacter baumannii is a Gram‐negative nosocomial pathogen of clinical importance. A lack of genetic tools has hindered the research of this organism in the past; however, recently, various methods have been designed, modified, and optimized to facilitate the genetic manipulation of A. baumannii . This unit describes some of the recent genetic advances and new recombinant tools developed for this pathogen, including standard transformation and conjugation techniques specifically developed for the bacteria. As the need to understand the basic biology of A . baumannii increases with the prospect of developing new therapeutics, the use of the basic genetic methods herein can provide the critical first step to identify genes required for infection. © 2014 by John Wiley & Sons, Inc.