UNIT 14B.10 Analysis of Human Papillomavirus Genome Replication Using Two‐ and Three‐Dimensional Agarose Gel Electrophoresis. Issue 1 (16th May 2017)

• Record Type: Journal Article
• Authors: Henno, Liisi; Tombak, Eva‐Maria; Geimanen, Jelizaveta; Orav, Marit; Ustav, Ene; Ustav, Mart
• Is Part Of: Current protocols in microbiology. Volume 45:Issue 1(2017)
• Page Start: 14B.10.1
• Page End: 14B.10.37
• Publication Date: 2017-05-16
• Subjects: two‐dimensional agarose gel electrophoresis -- three‐dimensional agarose gel electrophoresis -- HPV -- DNA replication -- minicircle production -- southern blot; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.28
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract : This unit includes the necessary information to conduct neutral/neutral and neutral/alkaline two‐dimensional and neutral/neutral/alkaline three‐dimensional agarose gel electrophoresis. The methodology has been optimized over the years to gain a better outcome from the hard‐to‐interpret signals of human papilloma virus replication intermediates obtained from two‐ and three‐dimensional agarose gels. Examples of typical results and interpretation of replication intermediate patterns are included, and the outcomes of multiple‐dimension assays are assessed using previously published experimental data. © 2017 by John Wiley & Sons, Inc.