UNIT 4C.2 Burkholderia thailandensis: Genetic Manipulation. Issue 1 (16th May 2017)

• Record Type: Journal Article
• Authors: Garcia, Erin C.
• Is Part Of: Current protocols in microbiology. Volume 45:Issue 1(2017)
• Page Start: 4C.2.1
• Page End: 4C.2.15
• Publication Date: 2017-05-16
• Subjects: allelic exchange -- conjugation -- mini‐Tn7 -- mutation -- transformation Burkholderia thailandensis; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.27
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract : Burkholderia thailandensis is a Gram‐negative bacterium endemic to Southeast Asian and northern Australian soils. It is non‐pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei . B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini‐Tn7 insertion, and allelic exchange in B. thailandensis . © 2017 by John Wiley & Sons, Inc.