UNIT 9.48 High Throughput‐Based Mitochondrial Function Assays by Multi‐Parametric Flow Cytometry. Issue 1 (1st July 2015)

• Record Type: Journal Article
• Authors: Robinson, J. Paul; Li, Nianyu; Narayanan, Padma Kumar
• Is Part Of: Current protocols in cytometry. Volume 73:Issue 1(2015)
• Page Start: 9.48.1
• Page End: 9.48.9
• Publication Date: 2015-07-01
• Subjects: mitochondria -- flow cytometry -- mitochondrial dysfunction -- mitochondrial membrane potential -- reactive oxygen species (ROS) -- cell viability; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/0471142956.cy0948s73
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract : Mitochondrial dysfunction has been increasingly implicated as an important mechanism for chemical‐induced toxicity. In the present unit, we describe a multi‐parametric flow cytometry assay to assess the effects of drug or chemical‐induced mitochondrial dysfunction in cells. Cells are cultured in a glucose‐supplemented medium and exposed to increasing concentrations of various chemicals. Several key mitochondrial/cellular parameters known to be directly impacted by mitochondrial dysfunction, such as mitochondrial membrane potential (MMP), mitochondrial reactive oxygen species (ROS) production, intracellular reduced glutathione (GSH) level, and cell viability, are simultaneously measured by flow cytometry. © 2015 by John Wiley & Sons, Inc.