Genotyping‐by‐Sequencing. Issue 1 (3rd March 2017)

• Record Type: Journal Article
• Authors: Wallace, Jason G.; Mitchell, Sharon E.
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 2:Issue 1(2017)
• Page Start: 64
• Page End: 77
• Publication Date: 2017-03-03
• Subjects: GBS -- genotyping -- high‐throughput sequencing -- RADseq -- restriction enzyme; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20042
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Genotyping‐by‐sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high‐throughput sequencing. GBS is a refinement of restriction site–associated DNA sequencing (RADseq) methods, with a goal of being able to perform library preparations quickly, cost‐effectively, and in a high‐throughput manner. This protocol contains the steps necessary to go from purified DNA to Illumina‐ready libraries. It also covers the considerations that go into planning a GBS experiment. © 2017 by John Wiley & Sons, Inc.