Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation. Issue 1 (27th April 2018)

• Record Type: Journal Article
• Authors: Vellanki, Sandeep; Navarro‐Mendoza, Maria Isabel; Garcia, Alexis; Murcia, Laura; Perez‐Arques, Carlos; Garre, Victoriano; Nicolas, Francisco E.; Lee, Soo Chan
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 49:Issue 1(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-04-27
• Subjects: gene knockout -- Mucor -- Mucor circinelloides -- mucormycosis -- RNAi; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.53
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a model organism for studying several biological processes, such as light responses and RNA interference–mediated gene silencing. Over the past decade, the increasing number of molecular tools has also allowed us to manipulate the genome of this fungus. This article outlines the fundamental protocols for the in vitro growth, maintenance, and genetic manipulation of M. circinelloides in the laboratory. © 2018 by John Wiley & Sons, Inc.