Culturing Rat Whole Ovary for UV Filter Benzophenone‐3 Treatment. Issue 1 (25th November 2019)

• Record Type: Journal Article
• Authors: Santamaría, Clarisa Guillermina; Abud, Julián Elías; Luque, Enrique Hugo; Kass, Laura; Rodríguez, Horacio Adolfo
• Editors: Bus, James S.; Costa, Lucio G.; Hodgson, Ernest; Lawrence, David A.; Reed, Donald J.
• Is Part Of: Current protocols in toxicology. Volume 82:Issue 1(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-11-25
• Subjects: organ culture -- ovary -- rat -- benzophenone‐3 -- rat whole ovary -- BP‐3; Toxicology -- Laboratory manuals; Toxicology -- methods; Toxicology; Laboratory Manual; Electronic books; Laboratory manuals; 571.95028
• DOI: 10.1002/cptx.89
• Languages: English
• ISSNs: 1934-9254

Abstract

Abstract: We describe a detailed protocol to establish a newborn rat whole ovary culture, which enables the study of direct effects (independent of hypothalamic‐pituitary‐gonadal axis) of endocrine disrupting chemicals (EDCs), such as benzophenone‐3 (BP‐3). This method is useful to understand changes in follicle formation, primordial to primary transition, and expression of regulatory molecules linked to these processes and also provides an alternative to animal models. © 2019 by John Wiley & Sons, Inc. Basic Protocol 1 : Rat ovarian surgery Basic Protocol 2 : Whole organ/ovarian culture Basic Protocol 3 : RNA isolation and quantitative real‐time PCR Basic Protocol 4 : Histological processing and staining