Amyloid β peptides are differentially vulnerable to preanalytical surface exposure, an effect incompletely mitigated by the use of ratios. Issue 1 (22nd March 2018)

• Record Type: Journal Article
• Authors: Toombs, Jamie; Foiani, Martha S.; Wellington, Henrietta; Paterson, Ross W.; Arber, Charles; Heslegrave, Amanda; Lunn, Michael P.; Schott, Jonathan M.; Wray, Selina; Zetterberg, Henrik
• Is Part Of: Alzheimer's & dementia. Volume 10:Issue 1(2018)
• Page Start: 311
• Page End: 321
• Publication Date: 2018-03-22
• Subjects: Alzheimer's disease -- Amyloid β ratio -- Preanalytical factors -- Cerebrospinal fluid -- Cell culture media -- Surface adsorption; Alzheimer's disease -- Periodicals; Alzheimer's disease -- Diagnosis -- Periodicals; Dementia -- Periodicals; Dementia -- Diagnosis -- Periodicals; 616.831
• DOI: 10.1016/j.dadm.2018.02.005
• Languages: English
• ISSNs: 2352-8729

Abstract

Abstract: Introduction: We tested the hypothesis that the amyloid β (Aβ) peptide ratios are more stable than Aβ42 alone when biofluids are exposed to two preanalytical conditions known to modify measurable Aβ concentration. Methods: Human cerebrospinal fluid (CSF) and culture media (CM) from human cortical neurons were exposed to a series of volumes and polypropylene surfaces. Aβ42, Aβ40, and Aβ38 peptide concentrations were measured using a multiplexed electrochemiluminescence immunoassay. Data were analyzed using mixed models in R. Results: Decrease of measurable Aβ peptide concentrations was exaggerated in longer peptides, affecting the Aβ42 :Aβ40 and Aβ42 :Aβ38 ratios. However, the effect size of surface treatment was reduced in Aβ peptide ratios versus Aβ42 alone. For Aβ42 :Aβ40, the effect was reduced by approximately 50% (volume) and 75% (transfer) as compared to Aβ42 alone. Discussion: Use of Aβ ratios, in conjunction with concentrations, may mitigate confounding factors and assist the clinical diagnostic process for Alzheimer's disease.