UNIT 15.4 ENU Mutagenesis in the Mouse. (15th July 2014)

• Record Type: Journal Article
• Authors: Stottmann, Rolf; Beier, David
• Is Part Of: Current protocols in human genetics. Volume 82(2014)
• Page Start: 15.4.1
• Page End: 15.4.10
• Publication Date: 2014-07-15
• Subjects: alkylating agent -- spermatogonial stem cells -- per locus mutation frequency -- phenotype‐driven mutagenesis -- genome sequencing; Human genetics -- Laboratory manuals; Genetic Techniques; Human genetics; Laboratory manuals; 599.935028
• DOI: 10.1002/0471142905.hg1504s82
• Languages: English
• ISSNs: 1934-8258

Abstract

ABSTRACT: This unit describes the treatment of laboratory mice with the mutagen N ‐ethyl‐ N ‐nitrosourea (ENU) to induce very highly increased rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations. Curr. Protoc. Hum. Genet . 82:15.4.1‐15.4.10 © 2014 by John Wiley & Sons, Inc.