Isolation and identification of novel l-Methioninase producing bacteria and optimization of its production by experimental design method. (July 2020)

• Record Type: Journal Article
• Authors: Mohkam, Milad; Taleban, Yasaman; Golkar, Nasim; Berenjian, Aydin; Dehshahri, Ali; Mobasher, Mohammad Ali; Ghasemi, Younes
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 26(2020)
• Publication Date: 2020-07
• Subjects: Alcaligenes -- Box-behnken -- Experimental design -- l-methioninase -- Optimization; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2020.101566
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: The current study deals with the production of l -methioninase from newly isolated bacterium Alcaligenes spp. via submerged fermentation. The statistical methods of one factor at a time (OFAT) and response surface methodology were used to optimize the nutritional and cultivation factors for the production of l -methioninase by Alcaligenes sp MT-B. The regression models depicted considerably high R 2 values of 0.87% for l -methioninase activities, signifying that they are suitable for forecasting associations between lactose, yeast extract, pH and temperature. In accordance with the model, the optimum condition for l -methioninase production were yeast extract 0.2%; lactose 0.75%, pH 6 and temperature 34.6 °C. These forecasted conditions were confirmed by validation trials. In the optimized condition Alcaligenes sp MT-B produced l -methioninase with the activity of 13.06 U/ml which is 1.4 times superior to that produced in initial fermentation condition. Hence, this statistical technique enabled fast identification and combination of key medium factors for Alcaligenes sp MT-B, resulted in the high l -methioninase production. Highlights: Introducing new bacterium genus ( Alcaligenes sp. MT-B) for l -methioninase production. Optimization of factors for l -methioninase production. l -methioninase could be used for cancer therapy.