Utilization of food waste-derived volatile fatty acids for production of edible Rhizopus oligosporus fungal biomass. (August 2020)

• Record Type: Journal Article
• Authors: Wainaina, Steven; Kisworini, Afrilia Dwi; Fanani, Marizal; Wikandari, Rachma; Millati, Ria; Niklasson, Claes; Taherzadeh, Mohammad J.
• Is Part Of: Bioresource technology. Volume 310(2020)
• Publication Date: 2020-08
• Subjects: Food waste -- Anaerobic digestion -- Volatile fatty acids -- Fed-batch cultivation -- Edible filamentous fungal biomass -- Rhizopus oligosporus; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123444
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Rhizopus oligosporus was grown solely on food waste-derived VFAs. Four-fold biomass increase observed in fed-batch compared to batch cultivation. Of the consumed carboxylates, acetic acid was the most preferred. Maximum biomass yield of 0.21 ± 0.01 g dry biomass/g VFAs COD eq. consumed obtained. Highest protein content in the biomass reached 39.28 ± 1.54%. Abstract: Rhizopus oligosporus is an edible filamentous fungus that can contribute to meet the growing demand for single-cell protein. Volatile fatty acids (VFAs) are favorable potential substrates for producing R. oligosporus biomass due to their capacity to be synthesized from a wide range of low-value organic solid wastes via anaerobic digestion. The goal of this work was to cultivate R. oligosporus using food waste-derived VFAs as the sole carbon source. To maintain the requisite low substrate concentrations, the fed-batch cultivation technique was applied. This resulted in a four-fold improvement in biomass production relative to standard batch cultivation. Maximum biomass yield of 0.21 ± 0.01 g dry biomass/g VFAs COD eq. consumed, containing 39.28 ± 1.54% crude protein, was obtained. In the bubble-column bioreactors, the complete uptake of acetic acid was observed, while the consumptions of caproic and butyric acids reached up to 97.64% and 26.13%, respectively.