Cross-linking/mass spectrometry to get a closer view on protein interaction networks. (June 2020)

• Record Type: Journal Article
• Authors: Iacobucci, Claudio; Götze, Michael; Sinz, Andrea
• Is Part Of: Current opinion in biotechnology. Volume 63(2020)
• Page Start: 48
• Page End: 53
• Publication Date: 2020-06
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2019.12.009
• Languages: English
• ISSNs: 0958-1669

Abstract

Abstract : Just recently, chemical cross-linking combined with mass spectrometry (XL-MS) has emerged as valuable tool to study protein interaction networks on the system-wide level. The current challenges in XL-MS are to develop robust workflows enabling a comprehensive capture of dynamic biological assemblies in their native environment in a routine manner. In this review, we will highlight both the latest technological developments as well as selected applications of XL-MS for investigating protein networks in cells, organisms, and tissue. In addition, different bioinformatics tools for data analysis will be presented. In light of these exciting new developments, XL-MS can be expected to become one of the most versatile techniques to address important biological questions in a timely manner.