Production of lipids and carotenoids by Rhodotorula gracilis ATCC 10788 yeast in a bioreactor using low-cost wastes. (July 2020)

• Record Type: Journal Article
• Authors: Kot, Anna M.; Błażejak, Stanisław; Kieliszek, Marek; Gientka, Iwona; Piwowarek, Kamil; Brzezińska, Rita
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 26(2020)
• Publication Date: 2020-07
• Subjects: Bioreactor -- Red yeast -- Single cell oil -- Microbial carotenoids -- Rhodotorula; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2020.101634
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: The study evaluated the possibility of using potato wastewater and glycerol fraction as media components for lipid and carotenoid biosynthesis by Rhodotorula gracilis ATCC 10788 yeast in a bioreactor. The cultures were carried out in 20 and 28 °C. A biomass yield range of up to 20 gd.w. /L was obtained after 72 h. The lipid content in yeast biomass was the highest (19 g/100 gd.w ) on the third and fourth days. After 96 h, it was observed that linoleic acid had the largest share (~28%). The biosynthesis of carotenoid by yeast cells grown in a bioreactor occurred for 120 h, but after taking into account the biomass yields, the highest value of the volumetric efficiency biosynthesis (6.24 mg/L) was found within 96 h of culture. After this time, the levels of β-carotene (~47%) and torulene (~51%) were similar, while torularhodin was synthesized in negligible amounts (<1%). Based on the obtained results, it was concluded that after cultivation in the bioreactor, the biomass of R. gracilis yeast could be a source of microbial lipids and carotenoids. Moreover, the reduction of chemical oxygen demand of the culture medium indicates that the proposed method of yeast cultivation may mitigate the environmental harmfulness of the used industrial waste biofuels and starch.