Application of the cellular oxidation biosensor to Toxicity Identification Evaluations for high-throughput toxicity assessment of river water. (May 2020)

• Record Type: Journal Article
• Authors: Ooi, Lia; Okazaki, Keisuke; Arias-Barreiro, Carlos R.; Heng, Lee Yook; Mori, Izumi C.
• Is Part Of: Chemosphere. Volume 247(2020)
• Publication Date: 2020-05
• Subjects: High-throughput cytotoxicity biosensor -- Toxicity identification evaluation -- River water pollution -- Ecotoxicity management -- Integrated watershed management; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.125933
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Toxicity Identification Evaluation (TIE) is a useful method for the classification and identification of toxicants in a composite environment water sample. However, its extension to a larger sample size has been restrained owing to the limited throughput of toxicity bioassays. Here we reported the development of a high-throughput method of TIE Phase I. This newly developed method was assisted by the fluorescence-based cellular oxidation (CO) biosensor fabricated with roGFP2-expressing bacterial cells in 96-well microplate format. The assessment of four river water samples from Langat river basin by this new method demonstrated that the contaminant composition of the four samples can be classified into two distinct groups. The entire toxicity assay consisted of 2338 tests was completed within 12 h with a fluorescence microplate reader. Concurrently, the sample volume for each assay was reduced to 50 μL, which is 600 to 4700 times lesser to compare with conventional bioassays. These imply that the throughput of the CO biosensor-assisted TIE Phase I is now feasible for constructing a large-scale toxicity monitoring system, which would cover a whole watershed scale. Highlights: A high-throughput cellular oxidation biosensor was applied to the TIE protocol. The newly developed TIE method is highly sensitive and rapid. The water sample volume was greatly reduced. The assay distinguished the toxicity profile in different water samples. A TIE protocol compatible with a large sample size is developed.