Bovine Bone Promotes Osseous Protection via Osteoclast Activation. (July 2020)

• Record Type: Journal Article
• Authors: Klein, Y.; Shani-Kdoshim, S.; Maimon, A.; Fleissig, O.; Levin-Talmor, O.; Meirow, Y.; Garber-Berkstein, J.; Leibovich, A.; Stabholz, A.; Chaushu, S.; Polak, D.
• Is Part Of: Journal of dental research. Volume 99:Number 7(2020)
• Page Start: 820
• Page End: 829
• Publication Date: 2020-07
• Subjects: bone regeneration -- osteoclastogenesis -- gene expression -- bone remodeling -- tissue healing -- histological healing; Dentistry -- Periodicals; Dentistry -- Social aspects -- Periodicals; Dentistry -- Periodicals; Research -- Periodicals; 617.6005
• DOI: 10.1177/0022034520911647
• Languages: English
• ISSNs: 0022-0345

Abstract

The current study aimed at investigating the long-term biological mechanisms governing bone regeneration in osseous defects filled with bovine bone (BB). Tooth extraction sockets were filled with BB or left unfilled for natural healing in a C57BL/6 mouse alveolar regeneration bone model ( n = 12). Seven weeks later, the alveolar bone samples were analyzed histologically with hematoxylin/eosin and tartrate-resistant acid phosphatase staining. A separate group ( n = 10) was used for RNA sequencing. Osteoclast inhibition was induced by zoledronic acid (ZA) administration at 2 wk postextraction in a third group ( n = 28) for examination of osseous changes and cellular functions with micro–computed tomography and quantitative reverse transcription polymerase chain reaction, respectively. Histological and radiological osseous healing was observed in both BB-filled and normal-healing sockets. However, BB regenerated bone showed significant robust expression of genes associated with bone homeostasis and osteoclasts' function. Osteoclasts' inhibition in BB-filled sockets led to decreased bone resorption markers and reduced bone formation to a greater extent than that observed in osteoclasts' inhibition with natural healing. BB displays long-term biologically active properties, despite a naive osseous histological appearance. These include activation of osteoclasts, which in turn promotes osseous remodeling and maturation of ossified bone.