Analysis of microRNA expression profiles in porcine PBMCs after LPS stimulation. Issue 5 (July 2020)

• Record Type: Journal Article
• Authors: Zhang, Jing; Xu, Xin; Huang, Xingfa; Zhu, Huiling; Chen, Hongbo; Wang, Wenjun; Liu, Yulan
• Is Part Of: Innate immunity. Volume 26:Issue 5(2020:Jul.)
• Page Start: 435
• Page End: 446
• Publication Date: 2020-07
• Subjects: PBMCs -- LPS -- microRNA -- small RNA sequencing -- pig; Natural immunity -- Periodicals; Endotoxins -- Periodicals; 616.07905
• DOI: 10.1177/1753425920901560
• Languages: English
• ISSNs: 1753-4259

Abstract

In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs. A total of 291 known miRNAs were bio-computationally identified in porcine PBMCs, and 228 novel miRNAs (not enlisted in the swine mirBase) were identified. Among these miRNAs, ssc-miR-148a-3p, ssc-let-7g, ssc-let-7f, 3_8760, ssc-miR-26a, ssc-miR-451, ssc-miR-21, ssc-miR-30d, ssc-miR-99a and ssc-miR-103 were the top 10 most abundant miRNAs in porcine PBMCs. Through miRNA differential analysis combined with quantitative PCR, we found the expressions of ssc-miR-122, ssc-miR-129b, ssc-miR-17-5p and ssc-miR-152 were significantly changed in porcine PBMCs after LPS stimulation. Furthermore, targets prediction and function analysis indicated a significant enrichment in gene ontology functional categories related to diseases, immunity and inflammation. In conclusion, this study on profiling of miRNAs expressed in LPS-stimulated PBMCs provides an important reference point for future studies on regulatory roles of miRNAs in porcine immune system.