Toxicological screening of airborne particulate matter in atmosphere of Pune: Reactive oxygen species and cellular toxicity. (June 2020)

• Record Type: Journal Article
• Authors: Jan, Rohi; Roy, Ritwika; Bhor, Renuka; Pai, Kalpana; Satsangi, P. Gursumeeran
• Is Part Of: Environmental pollution. Volume 261(2020)
• Publication Date: 2020-06
• Subjects: PM -- Redox active metals -- Cytotoxicity -- ROS; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2019.113724
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Present study screened the toxicological assessment of airborne particulate matter (PM), mechanistic investigation, relationship between the physicochemical characteristics and its associated toxic response. The average concentration of both PM10 and PM2.5 exceeded the Indian National Ambient Air Quality Standards. In present study, PM bound metals; Fe, Cu, Cr, Ni, Mn, Pb, Cd, Zn, Sr and Co have been taken into account with total metal concentration of 0.83 and 0.44 μg m −3 of PM10 and PM2.5 mass concentrations, respectively. The contribution of redox active metals (Fe, Cu, Cr, Ni and Mn) in PM was more as compared to non-redox metals (Pb, Cd and Co) indicating significant risk to the exposed population as these metals possess the ability to produce reactive oxygen species (ROS) which are responsible for various diseases. The cytotoxicity profiles of PM samples determined by MTT assay on two different cell lines (A549 and PBMC) exhibited dose-dependent effects after 24 h exposure, but the consequences differ with respect to particle size and sampling periods. A significant decrease in cell viability with varying PM concentrations (20, 40, 60, 80 and 100 μg ml −1 ) with respect to control was found in both cell lines. Incubation of RBC suspension with PM samples caused pronounced disruption of RBC and thus exhibited substantial hemolytic behavior. PM samples showed a range of potency to produce reactive oxygen species (ROS). Almost all PM samples increased the level of pro-inflammatory mediator (Nitric oxide) when compared to corresponding unexposed controls suggesting the important role of reactive nitrogen species in induction of cellular toxicity. Graphical abstract: Image 1 Highlights: PM bound metals were detected in the atmosphere of Pune. Fe exhibited the dominance amongst all analysed metals. A549 and PBMC cells exhibited concentration-dependent cytotoxic effects. DCFH-DA assay confirmed the oxidative nature of PM bound metals. PM samples caused pronounced disruption of RBC. Abstract : Present study highlights the toxicological assessment (in terms of ROS generation, NO-proinflammatory mediator, cell death and RBC hemolysis) of particulate matter in the atmosphere of Pune, India.