Evaluation of the cross-neutralization capacity of Thai green pit viper antivenom against venom of Myanmar green pit viper. (15th April 2020)

• Record Type: Journal Article
• Authors: Yee, Khin Than; Maw, Lwin Zar; Kyaw, Aung Myat; Khow, Orawan; Oo, Aye Win; Oo, Tin Ko Ko; Aung, Hsu Mon; Shwe, Thuzar Hla; Htwe, Su Myat; Myint, Zaw
• Is Part Of: Toxicon. Volume 177(2020)
• Page Start: 41
• Page End: 45
• Publication Date: 2020-04-15
• Subjects: Myanmar green pit viper venom -- Thai green pit viper antivenom -- Neutralization; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2020.02.003
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Green pit viper ( Trimeresurus sp.) bite occurred throughout Myanmar, but there is no specific antivenom produced in the country for related envenomation. Instead, Myanmar Russell's viper antivenom (Anti-MRV) was often misused because of prolonged clotting time was observed from both species. Thai green pit viper antivenom (Anti-TGPV) raised against Trimeresurus albolabris was found to be effective against venoms of more than ten Trimeresurus sp. from Thailand, Malaysia and Indonesia. The present study compared the neutralization capacities of Anti-TGPV and Anti-MRV towards the venom from T. erythrurus from Myanmar. Anti-TGPV was more efficacious than Anti-MRV in cross-neutralizing the lethal and haemorrhagic activities of the venom by a potency of a least 1.4 times higher. Although Anti-TGPV effectively cross-neutralized the coagulation activity of the venom, Anti-MRV failed to do so. Immunodiffusion and immunoblot experiments showed that Anti-TGPV cross-reacted with more protein components of the venom than Anti-MRV. In conclusion, Anti-TGPV is a better choice for patients bitten by Myanmar green pit viper, but further clinical investigation is required. The current findings highlight the development of a specific antivenom against Myanmar green pit viper venom. Highlights: LD50 for Myanmar green pit viper (MGPV) venom is 93.76 (74.64–116.68) μg/mouse. Thai green pit viper antivenom (Anti-TGPV) neutralizes MGPV venom better than Myanmar Russell's viper antivenom (Anti-MRV). Anti-TGPV can recognize more toxin proteins of Myanmar green pit viper venom than Anti-MRV. Anti-TGPV is a good choice for Myanmar green pit viper bite cases. Anti-MRV is obsolesce for Myanmar green pit viper bite cases.