UNIT 7.2 Immunoprecipitation. Issue 1 (1st June 2016)

• Record Type: Journal Article
• Authors: Bonifacino, Juan S.; Gershlick, David C.; Dell'Angelica, Esteban C.
• Is Part Of: Current protocols in cell biology. Volume 71: Issue 1(2016)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-06-01
• Subjects: immunoprecipitation -- immunoprecipitation‐recapture -- antigen -- antibody -- agarose bead -- magnetic bead; Cytology -- Laboratory manuals; Cytological Techniques; Cytology; Periodicals; 571.6028
• DOI: 10.1002/cpcb.3
• Languages: English
• ISSNs: 1934-2500

Abstract

Abstract : Selective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein‐protein interactions. Clear step‐by‐step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody. © 2016 by John Wiley & Sons, Inc.