Electrochemical detection of amyloid-β protein by delaminated titanium carbide MXene/multi-walled carbon nanotubes composite with molecularly imprinted polymer. (June 2020)

• Record Type: Journal Article
• Authors: Özcan, Nermin; Medetalibeyoglu, Hilal; Akyıldırım, Onur; Atar, Necip; Yola, Mehmet Lütfi
• Is Part Of: Materials today communications. Volume 23(2020)
• Publication Date: 2020-06
• Subjects: Molecular imprinting -- Amyloid-β protein -- Multi-walled carbon nanotubes -- Titanium carbide MXene -- Electrochemical bio Sensor; Materials science -- Periodicals; 620.11
• DOI: 10.1016/j.mtcomm.2020.101097
• Languages: English
• ISSNs: 2352-4928

Abstract

Highlights: Molecularly imprinted sensor based on delaminated titanium carbide MXene and multi-walled carbon nanotubes is developed for amyloid-β protein recognition. Molecularly imprinted sensor is characterized by several methods. Molecularly imprinted sensor shows high stability, repeatability, reproducibility and reusability. Abstract: Amyloid β-protein, a polypeptide, is known as the reason of neuronal death and is widely recognized as a biomarker of Alzheimer's disease. In this study, a simple and rapid electrochemical biosensor based on delaminated titanium carbide MXene (d-Ti3 C2 TX MXene) and multi-walled carbon nanotubes (MWCNTs) composite including molecularly imprinted polymers (MIPs) was formed for amyloid-β having 42-amino-acid-peptide (Aβ42 ) protein sensing. After d-Ti3 C2 TX /MWCNTs composite was prepared by mixing the colloidal solution of d-Ti3 C2 TX MXene with MWCNTs (mass ratio 3:1), some analytic methods such as x-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), UV/Vis spectroscopy, atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were performed for characterizations of d-Ti3 C2 TX MXene, MWCNTs and d-Ti3 C2 TX /MWCNTs composite. Later, Aβ42 imprinted d-Ti3 C2 TX /MWCNTs/GCE was prepared in a mixture including 100.0 mM pyrrole (monomer) containing 25.0 mM Aβ42 (template) in phosphate buffer (0.1 M, pH 7.5) by CV method for 25 cycles. Aβ42 imprinted electrode showed a linear range of 1.0 fg mL −1 - 100.0 fg mL -1 and detection limit (LOD) of 0.3 fg mL -1 were obtained. Aβ42 imprinted electrode was examined in terms of stability, repeatability, reproducibility and reusability. Finally, Aβ42 imprinted biosensor was applied to plasma samples for Aβ42 analysis.