Porous chitosan derivative scaffolds affect proliferation and osteogenesis of mesenchymal stem cell via reducing intracellular ROS. (1st June 2020)

• Record Type: Journal Article
• Authors: Wang, Jin; Zhou, Lehao; Sun, Qihao; Cai, Haibo; Tan, Wen-Song
• Is Part Of: Carbohydrate polymers. Volume 237(2020)
• Publication Date: 2020-06-01
• Subjects: Mesenchymal stem cell -- Chitosan -- Reactive oxygen species -- Osteogenic differentiation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116108
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: The N- polyphenol substituted chitosan derivatives (2a -2 g) were synthesized. The relationship between the structure and activity of 2a-2 g was analyzed. These 2a-2 g scaffolds had good biocompatibility, physicochemical properties. The 2 g scaffold had a positive effect on h ADSCs proliferation. The 2 g scaffold promoted osteogenesis of h ADSCs by reducing intracellular ROS. Abstract: The proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) needed to be promoted for their use in stem cell-based therapy for large bone defects. This study aimed to prepare porous 2a-2 g scaffolds with antioxidant activity to reduce intracellular reactive oxygen species (ROS), which resulted in promoting the proliferation and osteogenic differentiation of MSCs. A series of novel chemically modified 2a-2 g scaffolds were fabricated by an acid-soluble/alkali-insoluble method. Besides, these 2a-2 g scaffolds had good biocompatibility, physicochemical properties and the ability to promote osteogenic differentiation of human adipose-derived stem cells ( h ADSCs). However, the proliferation ability of h ADSCs on 2a-2f scaffolds was weakened. Interestingly, 2 g scaffold had a positive effect on h ADSCs proliferation. These results indicated that the reduction of intracellular ROS was not conducive to h ADSCs proliferation but beneficial to h ADSCs osteogenic differentiation. Taken together, these significant results highlighted potential therapeutic benefit of 2 g scaffold in large bone defects.