Structure of phosphorylated and sulfated polysaccharides from lipopolysaccharide of marine bacterium Marinicella litoralis KMM 3900T. (April 2020)

• Record Type: Journal Article
• Authors: Kokoulin, Maxim.S.; Lizanov, Igor N.; Romanenko, Lyudmila A.; Chikalovets, Irina V.
• Is Part Of: Carbohydrate research. Volume 490(2020)
• Publication Date: 2020-04
• Subjects: Marine bacterium -- Marinicella litoralis -- lipopolysaccharide -- Polysaccharide -- Methyl phosphate -- 3-O-Methyl-d-mannose; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2020.107961
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Two polysaccharide fractions were obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Marinicella litoralis KMM 3900 T . The major polysaccharide was found to contain glycerol 1-phosphate (PGro) and methyl phosphate substituents (PMe), and the following structure of its disaccharide repeating unit was established by sugar analysis, dephosphorylation, Smith degradation, and 1D and 2D NMR spectroscopy: →4)-α-L-Rha p 2PGro(~40%)-(1 → 3)-β-D-Man p 6PMe(~80%)-(1 → . The minor polysaccharide was shown to consist of 4- O -sulfate-d -mannopyranosyl residues, non-stoichiometric methylated at O-3 and acetylated at O-6: →2)-α-D-Man p 3R4S6Ac(~75%)-(1→, where R is Me (85%) or H (15%). Graphical abstract: Image 1 Highlights: Structures of two polysaccharides from M. litoralis KMM 3900 T were established. Major PS-1 was found to contain glycerol 1-phosphate and methyl phosphate substituents. Minor PS-2 was found to be a methylated and sulfated α-D-mannan.