Development of an in vitro system and model-based translational framework to assess haemolysis risk following intravenous abuse of medications containing polyethylene oxide. (June 2020)

• Record Type: Journal Article
• Authors: Persich, Peter; Engels, Gerwin E.; van Oeveren, Willem; Galia, Eric; Benay, Stéphan; Thun, Susanne
• Is Part Of: Toxicology in vitro. Volume 65(2020)
• Publication Date: 2020-06
• Subjects: Haemolysis -- Thrombotic microangiopathy -- Opioid abuse -- Polyethylene oxide -- Opana ER -- Translational; ER extended release -- FDA United States Food and Drug Administration -- G gauge -- MDa megadaltons -- OD optical density -- PEO polyethylene oxide -- TMA thrombotic microangiopathy; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2020.104776
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Multiple cases of potentially life-threatening thrombotic microangiopathy (TMA) have resulted from intravenous abuse of medications containing polyethylene oxide (PEO), most often Opana ER (oxymorphone hydrochloride extended release). No validated models are available to assess the risk of TMA with different formulations and extraction methods following intravenous abuse. We have developed an in vitro system that involves passing pooled blood containing the excipient of interest through a syringe needle and assessing haemolysis via haemoglobin release. Haemolysis is induced by high shear stress caused by the flow of blood containing PEO through a narrow-bore syringe needle, recapitulating the mechanism in small blood vessels. Using the in vitro system, we demonstrate that high-molecular-weight PEO (>1 MDa) induces haemolysis in a concentration-dependent manner under flowing but not static conditions. We use data from the in vitro system and published in vivo data to predict the time course of the haemolytic response in vivo via a pharmacometric model. The in vitro system is a novel method for investigating factors influencing PEO-induced haemolysis. In combination with our model-based translational framework, the in vitro system allows straightforward assessment of the haemolytic potential of PEO-containing medications, and may find application in gauging TMA risk following intravenous abuse. Highlights: Polyethylene oxide (PEO) is a common excipient in abuse-deterrent formulations. PEO can cause life-threatening thrombotic microangiopathy after intravenous abuse. PEO-induced haemolysis is the key initiating event in thrombotic microangiopathy. Our in vitro system measures haemolysis in flowing human blood containing PEO. Our model predicts the time course of haemolysis in vivo after injection of PEO.