Immunomodulatory activity of low molecular-weight peptides from Nibea japonica skin in cyclophosphamide-induced immunosuppressed mice. (May 2020)

• Record Type: Journal Article
• Authors: Yu, Fangmiao; He, Kang; Dong, Xiaoze; Zhang, Zhuangwei; Wang, Fenglei; Tang, Yunping; Chen, Yan; Ding, Guofang
• Is Part Of: Journal of functional foods. Volume 68(2020)
• Publication Date: 2020-05
• Subjects: Nibea japonica -- Protein hydrolysates -- Low molecular-weight peptide -- Immunosuppressed mice -- Immunomodulatory; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.103888
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: NJSP significantly helped mice to prevent the decrease of immunity. NJSP improved specific cellular immune function and humoral immune function in vivo . NJSP can potentially act as a promising immunomodulator. Abstract: In this study, a low molecular weight peptides fraction (<3 kDa, named NJSP) was isolated and purified from the skin protein hydrolysate of Nibea japonica by ultrafiltration. The in vivo immunomodulatory effects of NJSP were investigated in cyclophosphamide (CY)-induced immunosuppressed mice. The intragastric administration of NJSP (100, 200, 400 mg/kg/d) upregulated the immune organ indexes, restored the pathological histomorphology of immune organs, and improved the body weight of CY-treated mice in a time and dose-dependent manner. NJSP treatment also remarkably promoted the proliferation of splenocyte, enhanced phagocytosis of macrophages, restored delayed-type hypersensitivity (DTH). Moreover, NJSP administration dramatically improved hemolysin, tumor necrosis factor α (TNF-α), immunoglobulin (Ig) A, IgG and IgM levels in blood serum in a dose-dependent manner. Besides, NJSP also significantly increased the activities of superoxidase dismutase (SOD) and catalase (CAT), improved the total antioxidant capacity (T-AOC), and decreased malondialdehyde (MDA) levels in blood serum. These findings confirm that NJSP can protect the immune system against oxidative damage to maintain homeostasis. Finally, NJSP can serve as a promising immunomodulator with potential application in functional food and nutraceutical industry.