Novel strategy of incorporating biochar in solid-state fermentation for enhancing erythritol production by forming "microzones". (June 2020)

• Record Type: Journal Article
• Authors: Liu, Xiaoyan; Wang, Zhipeng; Yan, Yubo; Yu, Xinjun; Zhao, Pusu; Wang, Xiaoyu; Hu, Lei; Xu, Jiaxing; Xu, Jiming
• Is Part Of: Bioresource technology. Volume 306(2020)
• Publication Date: 2020-06
• Subjects: Biochar -- Erythritol production -- Solid state fermentation -- Batch fermentation -- Microzones; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123141
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Biochar was firstly applied in aerobic SSF for erythritol production. Cell–biochar–substrate "microzones" were supposed to be formed in biochar medium. An enhanced erythritol yield of 207.3 mg/gds was achieved with WSc addition. WSc in solid medium contributed well performed scale–up, fed and repeated–batch SSF. Abstract: Biochar is increasingly considered in addressing bioprocess issues due to its strong adsorbability and excellent compatibility to microbes. Here, biochar was first applied in aerobic solid-state fermentation (SSF) for erythritol production. Biochars derived from different agricultural wastes under various pyrolysis temperatures were evaluated, and wheat straw pyrolyzed at 300 °C (WSc) performed the best in enhancing fermentative erythritol production, with a dosage of 4% (w/w). In this procedure, cell–biochar–substrate "microzones" were formed, which was conductive to cell growth and attachment, and hence contributed enhanced enzyme activities, oil consumption, and erythritol production. The resultant erythritol productions of batch and fed-batch fermentations were 207.3 and 222.5 mg/gds, respectively. In repeated-batch fermentation, high cell viability and robust erythritol synthesis were maintained throughout seven cycles. This study demonstrates that SSF can be remarkably facilitated by biochar addition, suggesting a new perspective of biochar application in microbiological processes.