Comparative analysis of two brine shrimps revealed differential expression pattern and functional characterization of CK2α under bacterial stimulation from different geographical distribution. Issue 99 (April 2020)

• Record Type: Journal Article
• Authors: Wang, Zhangping; Hou, Qiru; Wan, Kun; Zhang, Rui; Dong, Lijun; Zhang, Daochuan; Yin, Hong
• Is Part Of: Fish & shellfish immunology. Issue 99(2020)
• Page Start: 631
• Page End: 640
• Publication Date: 2020-04
• Subjects: Artemia sinica -- Artemia parthenogenetica -- CK2α -- Bacterial stimulation different geographical distribution; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.02.047
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Understanding how the brine shrimp responds to different geographical populations can provide novel insights on response to bacterial stimulation. In the paper, Artemia sinica from lower altitudes and Artemia parthenogenetica from higher altitudes of the Tibetan Plateau, were used to illustrate different defense against bacteria mechanisms that these organisms used to adapt to different geographical environments. Protein kinase CK2 is a serine/threonine kinase with a multitude of protein substrates. It is a ubiquitous enzyme essential for the viability of eukaryotic cells, where its functions in a variety of cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection. The gene encodes the same mRNA sequence in A. sinica and A. parthenogenetica, named AsCK2α and ApCK2α, respectively. The open reading frame was obtained, a 1047-bp sequence encoding a predicted protein of 349 amino acids. To systematically analyze the expression of AsCK2α and ApCK2α during embryonic development and bacterial challenge, real-time PCR, Western blotting and immunohistochemistry were performed. The results showed that As CK2α was higher than Ap CK2α at different developmental stages. Under bacterial challenge, the expression of Ap CK2α was significantly higher than As CK2α. Protein localization analysis showed that As CK2α and Ap CK2α were mainly distributed in the head and chest. Our research revealed that CK2α plays a vital role in the growth, development and bacterial stimulation of the brine shrimp. Highlights: AsCK2α gene was identified and characterized in the brine shrimp for the first time. CK2α is differentially expressed of two brine shrimps at under bacterial stimulation. CK2α gene plays a vital role in the early development of the brine shrimp. CK2α may regulate the expression of immune related genes in the brine shrimp.